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Safety Practices for Working in the Biological Safety Cabinet and
in the Tissue Culture Room (or Suite)


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Transport of Biological Materials

Use of antimicrobial gel or wipe

When working with biological materials (e.g., infectious agents, human blood or materials, human cell lines, recombinant DNA molecules), a sink for handwashing should be present. When no sink is available, antimicrobial gel or wipe must be used after work followed by immediate handwashing in a sink.

Information on the gel or wipe
Description Size Fisher Cat. No.
BacdownGel Packaging 4 oz/30 x 4 oz case 1 gal/4 x 1 gal case 04-355-47 04-355-46
Bacdown AntimicrobialSkin Wipe Packaging 50 wipes/pk 04-355-60

Use of Bunsen burner with pilot light

When working in a BSC with a valid certification, use of a Bunsen burner is not necessary and thus for the safety reason, is not recommended. However, if it is used, only Touch-O-Matic Bunsen burner with a pilot light (Fisher Cat. No. 03-975) is allowed in the BSC and a full flame must never be left unattended even for a brief period of time.

Protection of vacuum line

When working with biological materials, protection of a vacuum line is required between the trap bottle and the vacuum nozzle. This can be achieved by inserting a 0.22 mm filter.


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